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Laboratory for Functional Genome Analysis

 

 

 

 

 


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Proteomics

LAFUGAs proteomics group has state-of-the-art equipment for gel-based as well as LC-MS-based proteomics.

LC-MS-based proteomics:
Ettan MDLC 2-dimensional nanoflow HPLC (GE Healthcare)

nanoACQUITY UPLC System (Waters)
EASY-nLC 1000 (Thermo)
Eksigent Ultra (SCIEX)
Dionex - UltiMate 3000 Nano LC System (Thermo)

Mass Spectrometry:
LTQ Linear Ion Trap ESI mass spectrometer (Thermo)
LTQ Orbitrap XL ESI mass spectrometer (Thermo)
5500 Hybrid Triple Quadrupole Linear Ion Trap (SCIEX)
TripleTOF 5600+ (Sciex)

Prefractionation:
Mutidimensional HPLC BioCAD Vision (Applied Biosystems)
3100 OFFGEL Fractionator (Agilent)

Sample Preparation:
PROTEINEER spII Automated Spot Picker (Bruker Daltonics)

DigestPro MS digester (Intavis)

Gel-based separation:
Several electrophoresis systems (Multiphor, IPGphor, Ettan DALT)
Typhoon fluorescence scanner with 3 Lasers for 2D-DIGE (GE Healthcare)
Proteomeweaver 2D-gel analysis software (Definiens)
DeCyder 2-D Differential Analysis Software (Amersham Biosciences)

Peptide Synthesis:
433A peptide synthesizer (Applied Biosystems)
Multiple peptide synthesizer (Abimed)
SYRO spot synthesizer (Multisyntech)