L   A   F   U   G   A

Laboratory for Functional Genome Analysis















The Munich MIDY Pig Biobank - A unique resource for studying organ crosstalk in diabetes. Blutke A, Renner S, Flenkenthaler F, Backman M, Haesner S, Kemter E, Ländström E, Braun-Reichhart C, Albl B, Streckel E, Rathkolb B, Prehn C, Palladini A, Grzybek M, Krebs S, Bauersachs S, Bähr A, Brühschwein A, Deeg CA, De Monte E, Dmochewitz M, Eberle C, Emrich D, Fux R, Groth F, Gumbert S, Heitmann A, Hinrichs A, Keßler B, Kurome M, Leipig-Rudolph M, Matiasek K, Öztürk H, Otzdorff C, Reichenbach M, Reichenbach HD, Rieger A, Rieseberg B, Rosati M, Saucedo MN, Schleicher A, Schneider MR, Simmet K, Steinmetz J, Übel N, Zehetmaier P, Jung A, Adamski J, Coskun Ü, Hrabě de Angelis M, Simmet C, Ritzmann M, Meyer-Lindenberg A, Blum H, Arnold GJ, Fröhlich T, Wanke R, Wolf E. Mol Metab. 2017 Jun 13;6(8):931-940. doi: 10.1016/j.molmet.2017.06.004. eCollection 2017 Aug.

Antibodies against the mono-methylated arginine-glycine repeat (MMA-RG) of the Epstein-Barr virus nuclear antigen 2 (EBNA2) identify potential cellular proteins targeted in viral transformation. Ayoubian H, Fröhlich T, Pogodski D, Flatley A, Kremmer E, Schepers A, Feederle R, Arnold GJ, Grässer FA. J Gen Virol. 2017 Jul 28. doi: 10.1099/jgv.0.000870. [Epub ahead of print]

Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease - UMOD (ADTKD-UMOD). Kemter E, Fröhlich T, Arnold GJ, Wolf E, Wanke R. Sci Rep. 2017 Feb 21;7:42970. doi: 10.1038/srep42970

Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins. Nynca J, Arnold GJ, Fröhlich T, Ciereszko A. Proteomics. 2017 Jun;17(11). doi: 10.1002/pmic.201600460.

ADNP is a therapeutically inducible repressor of WNT signaling in colorectal cancer. Blaj C, Bringmann A, Schmidt EM, Urbischek M, Lamprecht S, Fröhlich T, Arnold GJ, Krebs S, Blum H, Hermeking H, Jung A, Kirchner T, Horst D. Clin Cancer Res. 2016 Nov 30. pii: clincanres.1604.2016. [Epub ahead of print]


ROS-Mediated Inhibition of S-nitrosoglutathione Reductase Contributes to the Activation of Anti-oxidative Mechanisms. Kovacs I, Holzmeister C, Wirtz M, Geerlof A, Fröhlich T, Römling G, Kuruthukulangarakoola GT, Linster E, Hell R, Arnold GJ, Durner J, Lindermayr C. Front Plant Sci. 2016 Nov 10;7:1669.

Improved cryotolerance and developmental potential of in vitro and in vivo matured mouse oocytes by supplementing with a glutathione donor prior to vitrification. Trapphoff T, Heiligentag M, Simon J, Staubach N, Seidel T, Otte K, Fröhlich T, Arnold GJ, Eichenlaub-Ritter U. Mol Hum Reprod. 2016 Sep 7. [Epub ahead of print]

Modelling oviduct fluid formation in vitro. Simintiras CA, Fröhlich T, Sathyapalan T, Arnold GJ, Ulbrich SE, Leese HJ, Sturmey RG. Reproduction. 2016 Oct 13. pii: REP-15-0508. [Epub ahead of print]

Progressive muscle proteome changes in a clinically relevant pig model of Duchenne muscular dystrophy. Fröhlich T, Kemter E, Flenkenthaler F, Klymiuk N, Otte KA, Blutke A, Krause S, Walter MC, Wanke R, Wolf E, Arnold GJ. Sci Rep. 2016 Sep 16;6:33362. doi: 10.1038/srep33362.

Cyclin-dependent kinase 5 stabilizes hypoxia-inducible factor-1α: a novel approach for inhibiting angiogenesis in hepatocellular carcinoma. Herzog J, Ehrlich SM, Pfitzer L, Liebl J, Fröhlich T, Arnold GJ, Mikulits W, Haider C, Vollmar AM, Zahler S. Oncotarget. 2016 May 10;7(19):27108-21. doi: 10.18632/oncotarget.8342.

Postovulatory aging affects dynamics of mRNA, expression and localization of maternal effect proteins, spindle integrity and pericentromeric proteins in mouse oocytes. Trapphoff T, Heiligentag M, Dankert D, Demond H, Deutsch D, Fröhlich T, Arnold GJ, Grümmer R, Horsthemke B, Eichenlaub-Ritter U. Hum Reprod. 2016 Jan;31(1):133-49. doi: 10.1093/humrep/dev279.

LC-MS/MS analysis reveals a broad functional spectrum of proteins in the secretome of sebocytes. Dahlhoff M, Fröhlich T, Arnold GJ, Zouboulis CC, Schneider MR. Exp Dermatol. 2016 Jan;25(1):66-7. doi: 10.1111/exd.12867.


The influence of simulated microgravity on the proteome of Daphnia magna. Trotter B, Otte KA, Schoppmann K, Hemmersbach R, Fröhlich T, Arnold GJ & Laforsch C npj Microgravity 1, Article number: 15016 (2015)

Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules. Windschüttl S, Kampfer C, Mayer C, Flenkenthaler F, Fröhlich T, Schwarzer JU, Köhn FM, Urbanski H, Arnold GJ, Mayerhofer A. 2015 Sci Rep. 5:12820.

Interclonal proteomic responses to predator exposure in Daphnia magna may depend on predator composition of habitats. Otte KA, Schrank I, Fröhlich T, Arnold GJ, Laforsch C. 2015 Mol Ecol. (15):3901-17.

Proteomic analysis of extracellular medium of cryopreserved carp (Cyprinus carpio L.) semen. Dietrich MA, Arnold GJ, Fröhlich T, Otte KA, Dietrich GJ, Ciereszko A. Comp Biochem Physiol Part D Genomics Proteomics. 2015 15:49-57.

Betacellulin transgenic mice develop urothelial hyperplasia and show sex-dependent reduction in urinary major urinary protein content. Schulz H, Dahlhoff M, Glogowska A, Zhang L, Arnold GJ, Fröhlich T, Schneider MR, Klonisch T. 2015 Exp Mol Pathol. 99(1):33-8.

Readthrough acetylcholinesterase (AChE-R) and regulated necrosis: pharmacological targets for the regulation of ovarian functions? Blohberger J, Kunz L, Einwang D, Berg U, Berg D, Ojeda SR, Dissen GA, Fröhlich T, Arnold GJ, Soreq H, Lara H, Mayerhofer A. 2015 Cell Death Dis. 6,e1685

Cryopreservation-induced alterations in protein composition of rainbow trout semen. Nynca J, Arnold GJ, Fröhlich T, Ciereszko A. 2015 Proteomics, (15):2643-54

Characterization of the Sebocyte Lipid Droplet Proteome Reveals Novel Potential Regulators of Sebaceous Lipogenesis. Dahlhoff M, Fröhlich T, Arnold GJ, Müller U, Leonhardt H, Zouboulis CC, Schneider MR. 2015 Exp Cell Res., 332(1):146-55


Shotgun proteomics of rainbow trout ovarian fluid. Nynca J, Arnold GJ, Fröhlich T, Ciereszko A. 2014 Reprod Fertil Dev., [Epub ahead of print]

In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa. Dietrich MA, Arnold GJ, Fröhlich T, Ciereszko A. 2014 Comp Biochem Physiol Part D Genomics Proteomics, 12:10-5

Proteome analysis of early lineage specification in bovine embryos. Demant M, Deutsch DR, Fröhlich T, Wolf E, Arnold GJ.2014 Proteomics. 15(4):688-701

Stage-specific proteome signatures in early bovine embryo development. Deutsch DR, Fröhlich T, Otte KA, Beck A, Habermann FA, Wolf E, Arnold GJ. 2014 J Proteome Res.  13(10):4363-76.

Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses. Otte KA, Fröhlich T, Arnold GJ, Laforsch C. 2014 BMC Genomics 15:306.

Proteomic identification of rainbow trout sperm proteins. Nynca J, Arnold GJ, Fröhlich T, Otte K, Ciereszko A. 2014 Proteomics, 14(12):1569-73.

Characterization of carp seminal plasma proteome in relation to blood plasma. Dietrich MA, Arnold GJ, Nynca J, Fröhlich T, Otte K, Ciereszko A. 2014 J Proteomics,  98:218-32.

Secretome Analysis of Testicular Peritubular Cells: A Window into the Human Testicular Microenvironment and the Spermatogonial Stem Cell Niche in Man. Flenkenthaler F, Windschüttl S, Frohlich T, Schwarzer JU, Mayerhofer A, Arnold GJ. 2014 J Proteome Res.13(3):1259-69.

Proteomic identification of rainbow trout seminal plasma proteins. Nynca J, Arnold GJ, Fröhlich T, Otte K, Flenkenthaler F, Ciereszko A. 2014 Proteomics, (1):133-40.

V-ATPase inhibition by archazolid leads to lysosomal dysfunction resulting in impaired cathepsin B activation in vivo. Kubisch R, Fröhlich T, Arnold GJ, Schreiner L, von Schwarzenberg K, Roidl A, Vollmar A, Wagner E. 2014 Int J Cancer.  134(10):2478-88.


iTRAQ proteome analysis reflects a progressed differentiation state of epiblast derived versus inner cell mass derived murine embryonic stem cells. Fröhlich T, Kösters M, Graf A, Wolf E, Kobolak J, Brochard V, Dinnyés A, Jouneau A, Arnold GJ. 2013 J Proteomics. 90:38-51.

ATP-competitive LRRK2 inhibitors interfere with monoclonal antibody binding to the kinase domain of LRRK2 under native conditions. A method to directly monitor the active conformation of LRRK2? Gillardon F, Kremmer E, Froehlich T, Ueffing M, Hengerer B, Gloeckner CJ. 2013 J Neurosci Methods. 214(1):62-8. 

Promiscuous behaviour of archaeal ribosomal proteins: Implications for eukaryotic ribosome evolution.
Armache JP, Anger AM, Márquez V, Franckenberg S, Fröhlich T, Villa E, Berninghausen O, Thomm M, Arnold GJ, Beckmann R, Wilson DN. 
2013 Nucleic Acids Res. 41(2):1284-93

2-D DIGE analysis of the bursa of Fabricius reveals characteristic proteome profiles for different stages of chicken B-cell development.
Korte J, Fröhlich T, Kohn M, Kaspers B, Arnold GJ, Härtle S. 2013 Proteomics13(1):119-33.


First inducible transgene expression in porcine large animal models.
Klymiuk N, Böcker W, Schönitzer V, Bähr A, Radic T, Fröhlich T, Wünsch A, Keßler B, Kurome M, Schilling E, Herbach N, Wanke R, Nagashima H, Mutschler W, Arnold GJ, Schwinzer R, Schieker M, Wolf E.
2012 FASEB J.  26(3):1086-99.

2D DIGE Saturation Labeling for Minute Sample Amounts. Arnold GJ, Fröhlich T 2012 Methods Mol Biol.  854:89-112.

Vitrification at the pre-antral stage transiently alters inner mitochondrial membrane potential but proteome of in vitro grown and matured mouse oocytes appears unaffected. Demant M, Trapphoff T, Fröhlich T, Arnold GJ, Eichenlaub-Ritter U. 2012 Hum Reprod.  27(4):1096-111

A new strategy for the development of monoclonal antibodies for the determination of human procalcitonin in serum samples. Kremmer E, Meyer K, Grässer FA, Flatley A, Kösters M, Luppa PB, Krämer PM. 2012 Anal Bioanal Chem., 402(2):989-95.


Human tryptase cleaves pro-nerve growth factor (PRONGF): hints of a local, mast cell-dependent regulation of NGF/PRONGF action. Spinnler K., Frohlich T., Arnold G.J., Kunz L., Mayerhofer A.. 2011 J Biol Chem., 286(36):31707-13.

Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring (SRM), Fröhlich T, Arnold GJ
2011 Methods Mol Biol., 790:141-64.

The Ecoresponsive Genome of Daphnia pulex. Colbourne JK., Pfrender ME, Gilbert D, Thomas WK, Tucker A, Oakley TH, Tokishita S, Aerts A, Arnold GJ, Basu MK, Bauer DJ, Cáceres CE, Carmel L, Casola C, Choi J-H, Detter JC, Dong Q, Dusheyko S, Eads BD, Frohlich T,Geiler-Samerotte KA, Gerlach D, Hatcher P, Jogdeo S, Krijgsveld J, Kriventseva EV, Kültz D, Laforsch C, Lindquist E, Lopez J, Manak JR, Muller J, Pangilinan J, Patwardhan RP, Pitluck S, Pritham EJ, Rechtsteiner A, Rho M, Rogozin IB, Sakarya O, Salamov A, Schaack S, Shapiro H, Shiga Y, Skalitzky C, Smith Z, Souvorov A, Sung W, Tang Z, Tsuchiya D, Tu H, Vos H, Wang M, Wolf YI, Yamagata H, Yamada T, Ye Y, Shaw JR, Andrews J, Crease TJ, Tang H, Lucas SM, Robertson HM, Bork P, Koonin EV, Zdobnov EM, Grigoriev IV, Lynch M, Boore JL., 2011 Science 331 555-561.

Proteomic characterization of archaeal ribosomes reveals the presence of novel archaeal-specific ribosomal proteins. Márquez V*, Fröhlich T*, Armache JP, Sohmen D, Dönhöfer A, Mikolajka A, Berninghausen O, Thomm M, Beckmann R, Arnold GJ, Wilson DN. 2011 J Mol Biol. 405(5):1215-32. * Contributed equally

Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy. Ulbrich SE, Meyer SU, Zitta K, Hiendleder S, Sinowatz F, Bauersachs S, Büttner M, Frohlich T, Arnold GJ, Reichenbach HD, Wolf E, Meyer HH. 2011 Mol Cell Endocrinol 332(1-2):48-57.

Dynamic proteome signatures in gametes, embryos and their maternal environment. Arnold G.J., Frohlich T. 2011, Reproduction, Fertility and Development, 23, 1, 81-93


Identification of novel downstream targets of platelet glycoprotein VI activation by differential proteome analysis: implications for thrombus formation. Schulz C, Leuschen NV, Frohlich T, Lorenz M, Pfeiler S, Gleissner CA, Kremmer E, Kessler M, Khandoga AG, Engelmann B, Ley K, Massberg S, Arnold GJ. 2010 BLOOD 115 20

Creating new knowledge for ruminant reproduction from rapidly expanding and evolving scientific databases. Bauersachs S, Blum H, Krebs S, Fröhlich T, Arnold GJ, Wolf E. Soc Reprod Fertil Suppl. 2010;67:29-40.

In vivo chemoresistance of prostate cancer in metronomic cyclophosphamide therapy. Thoenes L, Hoehn M, Kashirin R, Ogris M, Arnold GJ, Wagner E, Guenther M. J Proteomics. 2010 May 7;73(7):1342-54.


A Newcomer's Guide to Nano-Liquid-Chromatography of Peptides. Frohlich T, Arnold GJ. 2009: Methods Mol Biol, 564 123-141.

LC-MS/MS-based proteome profiling in Daphnia pulex and Daphnia longicephala: the Daphnia pulex genome database as a key for high throughput proteomics in Daphnia. Frohlich T; Arnold GJ; Fritsch R; Mayr T; Laforsch C, 2009 BMC Genomics, 10 171.

Evidence for Estrogen-Dependent Uterine Serpin (SERPINA14) Expression During Estrus in the Bovine Endometrial Glandular Epithelium and Lumen. Ulbrich SE; Frohlich T; Schulke K; Englberger E; Waldschmitt N; Arnold GJ; Reichenbach HD; Reichenbach M; Wolf E; Meyer HH; Bauersachs S., 2009 Biol Reprod, 3 3.

Highly sensitive saturation labeling reveals changes in abundance of cell cycle-associated proteins and redox enzyme variants during oocyte maturation in vitro. Berendt FJ*; Frohlich T*; Bolbrinker P; Boelhauve M; Gungor T; Habermann FA; Wolf E; Arnold GJ, 2009 Proteomics, 9 550-564. * contributed equally


The brain proteome profile is highly conserved between Prnp-/- and Prnp+/+ mice. Crecelius A. C., Götz A, Arzberger T, Fröhlich T, Arnold GJ, Ferrer I, Kretzschmar HA. 200: Neuroreport, 19 1027-1031.

Single chain Fv antibodies directed against the 37 kDa/67 kDa laminin receptor as therapeutic tools in prion diseases. Zuber C.; Knackmuss S.; Rey C.; Reusch U.; Rottgen P.; Frohlich T.; Arnold G. J.; Pace C.; Mitteregger G.; Kretzschmar H. A.; Little M.; Weiss S., 2008 Molecular Immunology, 45 144-151.

Assessing quantitative post-mortem changes in the gray matter of the human frontal cortex proteome by 2-D DIGE. Crecelius A; Gotz A; Arzberger T; Frohlich T; Arnold GJ; Ferrer I; Kretzschmar H. A., 2008 Proteomics, 8 1276-1291.


Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants. Helm M; Luck C; Prestele J; Hierl G; Huesgen PF; Froehlich T; Arnold GJ; Adamska I.; Gorg A.; Lottspeich F.; Gietl C., 2007 Proceedings of the National Academy of Sciences of the United States of America, 104 11501-11506.


Proteome research based on modern liquid chromatography - tandem mass spectrometry: separation, identification and quantification. Frohlich T; Arnold GJ, 2006 Journal of Neural Transmission, 113 973-994.

Analysis of the HUPO Brain Proteome reference samples using 2-d DIGE and 2-d LC-MS/MS. Frohlich T; Helmstetter D.; Zobawa M.; Crecelius A. C.; Arzberger T.; Kretzschmar H. A.; Arnold GJ, 2006 Proteomics, 6 4950-4966.

Methods for transcriptome and proteome research: Applications for studying biology of reproduction in bovine. Wolf E; Hiendleder S; Bauersachs S; Frohlich T; Sinowatz F; Blum H; Arnold GJ, 2006 Berliner und Munchener Tierarztliche Wochenschrift, 119 7-16.

HUPO Brain Proteome Project: summary of the pilot phase and introduction of a comprehensive data reprocessing strategy. Hamacher M.; Apweiler R.; Arnold GJ; Becker A.; Bluggel M.; Carrette O.; Colvis C.; Dunn M. J.; Frohlich T; Fountoulakis M.; van Hall A.; Herberg F.; Ji J.; Kretzschmar H.; Lewczuk P.; Lubec G.; Marcus K.; Martens L.; Palacios Bustamante N.; Park Y. M.; Pennington S. R.; Robben J.; Stuhler K.; Reidegeld K. A.; Riederer P.; Rossier J.; Sanchez J. C.; Schrader M.; Stephan C.; Tagle D.; Thiele H.; Wang J.; Wiltfang J.; Yoo J. S.; Zhang C.; Klose J.; Meyer H. E., 2006 Proteomics, 6 4890-4898.

Functional genome research - new strategies to improve fertility in cattle. Wolf E; Arnold GJ; Bauersachs S; Blum H; Frohlich T; Hiendleder S.; Mitko K.; Reichenbach H. D., 2006 Zuchtungskunde, 78 428-439.

Before 2006

DRO1, a gene down-regulated by oncogenes, mediates growth inhibition in colon and pancreatic cancer cells. Bommer G. T.; Jager C. J.; Durr E. M.; Baehs S.; Eichhorst S. T.; Brabletz T.; Hu G.; Frohlich T; Arnold GJ; Kress D. C.; Goke B.; Fearon E. R.; Kolligs F. T., 2005 Journal of Biological Chemistry, 280 7962-7975.

Holistic differential analysis of embryo-induced alterations in the proteome of bovine endometrium in the preattachment period. Berendt FJ *; Frohlich T *; Schmidt S. E. M.; Reichenbach H. D.; Wolf E.; Arnold GJ, 2005 Proteomics, 5 2551-2560. * contributed equally

Functional genomics: tools for improving farm animal health and welfare. Hiendleder S; Bauersachs S; Boulesteix A; Blum H; Arnold GJ; Frohlich T; Wolf E., 2005 Revue Scientifique et Technique-Office International des Epizooties, 24 355-377.

Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein - Absolute quantification using isotope-coded tags and mass spectrometry. Hochleitner EO; Kastner B.; Frohlich T; Schmidt A.; Luhrmann R.; Arnold GJ; Lottspeich F., 2005: Journal of Biological Chemistry, 280 2536-2542.

Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material. Nicholls J.; Hahn P.; Kremmer E.; Frohlich T; Arnold GJ; Sham J.; Kwong D.; Grasser F. A., 2004 Journal of Virological Methods, 116 79-88.

Embryo-maternal communication in bovine - Strategies for deciphering a complex cross-talk. Wolf E; Arnold GJ; Bauersachs S.; Beier H. M.; Blum H.; Einspanier R.; Frohlich T; Herrler A.; Hiendleder S.; Kolle S.; Prelle K.; Reichenbach H. D.; Stojkovic M.; Wenigerkind H.; Sinowatz F., 2003 Reproduction in Domestic Animals, 38 276-289.

Overexpression of insulin-like growth factor-binding protein-2 in transgenic mice reduces postnatal body weight gain. Hoeflich A.; Wu M. Y.; Mohan S.; Foll J.; Wanke R.; Froehlich T; Arnold GJ; Lahm H.; Kolb H. J.; Wolf E., 1999 Endocrinology, 140 5488-5496.